As the temperature drops, extracellular water begins to freeze, leaving behind a slush of concentrated solutes. In an attempt to dilute those solutes, water rushes out of the cell (3), causing ...

This process allows cells to continue growing and prevents overcrowding and nutrient depletion. Cryopreservation is the process of freezing cells at ultra-low temperatures (typically -196 °C in liquid ...

is used to freeze samples in milliseconds without significant ice crystal damage. Chemical fixation at room temperature takes order of magnitude longer because it takes that long for fixatives to ...

Grow the MEF cultures to confluency, collect the cells and freeze (one vial/flask ... or if you are unfamiliar with these techniques, it is advisable to follow the sequence for preparing feeder ...