coli DNA ligase that she had obtained from Paul Modrich ... along with their drug-resistance plasmid as a new, improved cloning vector to show that frog DNA could also be replicated and transcribed in ...

A plasmid is a small, circular, double-stranded DNA molecule that is distinct from a cell's chromosomal DNA. Plasmids naturally exist in bacterial cells, and they also occur in some eukaryotes.

The cutting and joining manipulations that underlies gene cloning are carried out by enzymes called restriction endonucleases (for cutting) and ligases (for joining ... cut the insert (=the gene) from ...

Scientists in The Wistar Institute lab of David B. Weiner, Ph.D., have described a next-generation vaccination technology ...