1. You will use known amounts of the reaction product p-nitrophenol (in citrate buffer) to obtain a standard curve. The stock solution provided is 10 µmol/ml. As p-nitrophenol is a skin irritant, you ...

The first step of the assay development was an enzyme titration performed to obtain ... Using the inhibition curve (Fig. 3), we calculated the half-maximal inhibitory concentration (IC 50) to ...