As the temperature drops, extracellular water begins to freeze, leaving behind a slush of concentrated solutes. In an attempt to dilute those solutes, water rushes out of the cell (3), causing ...
Grow the MEF cultures to confluency, collect the cells and freeze (one vial/flask) as described in Steps 16–20 These are passage-zero MEFs. Split each flask (from Step 3) 1:5 to generate 25 ...
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