Blood Titers - Everything You Need To Know About Blood Titers
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Plaque Assay: A Method to Determine Viral Titer as Plaque Forming Units (PFU)
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SOLVED: To determine the viral titer of a T4 bacteriophage sample, you start with a serial dilution of your sample in dilution tubes from 10^4 to 10^7 dilution factor. Then you plate out a sample volume of 100 ul, incubated in susceptible E. coli strain by mixing the virus-infected cultures with top agar and pouring it over the solidified NA plate. Only the plate produced from the 10^-6 dilution tube has the required number of plaques to be able to calculate the original titer. You count 125 pla
SOLVED: To determine the viral titer of a T4 bacteriophage sample, yo…
Nov 2, 2021
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